Investigation on Post Translational Modification of GSK3 Beta Upon SIRT6 Silencing in Non-Small Cell Lung Cancer Cell Lines

Abstract

SIRT6 aberration has been widely reported in a variety of serious human disorders during the past few decades.This work is to analyse the SIRT6 knockdown effect on the stability of a non-small cell lung cancer cell lines expressing GSK-3Beta.The main objectives includes Silencing of SIRT6 in A549 and H460 cell lines by using (SIRT6 siRNA 1, SIRT6 siRNA 2) and investigation on the phosphorylation status of GSK-3beta.Methods used in this work are In vitro experiments with human NSCLC cells have been performed. Western Blot was performed to explore the key events in the regulation of GSK-3β by silencing SIRT6 in NSCLC. The findings of this study suggest that silencing of SIRT6 significantly promotes the phosphorylation status of GSK-3β and destabilizes it.